Chlorophytum borivilianum pdf

  1. In vitro multiplication of Chlorophytum borivilianum Sant. and Fern
  3. Chlorophytum in Flora of China @
  4. Chlorophytum borivilianum: A White Gold for Biopharmaceuticals and Neutraceuticals

PDF | Chlorophytum borivilianum Santapau & Fernandes (Liliaceae) also known as 'Safed Musli' is a traditional rare Indian medicinal herb which has many. PDF | Chlorophytum borivilianum Santapau & Fernandes (Liliaceae) also known as `Safed Musli' is a traditional rare Indian medicinal herb which has many. Chlorophytum borivilianum (Safed musli): A Vital Herbal Drug. Payal Sharma*, Kaushal K Chandrul. School of Pharmaceutical Science, Shri Venkateshwara.

Language:English, Spanish, Japanese
Genre:Academic & Education
Published (Last):30.03.2016
Distribution:Free* [*Sign up for free]
Uploaded by: KATHARYN

51320 downloads 149844 Views 22.70MB PDF Size Report

Chlorophytum Borivilianum Pdf

were higher in diabetic rats receiving C. borivilianum root extract treatment. C. borivilianum Safed Musli or Chlorophytum borivilianum (family. Safed musli (Chlorophytum borivilianum) is an eminent medicinal plant of India and considered as a 'white gold' or 'divya aushad' in Indian. INTRODUCTION. Safed musli (Chlorophytum borivilianum) is a herb, belongs to family Liliaceae. It was originally grown in thick forests of India. About.

Prasad , P. Its roots tubers are widely used for various therapeutic applications. It is used to cure physical illness and weakness, as an aphrodisiac agent and revitalizer, as general sex tonic, remedy for diabetes, arthritis and increasing body immunity, curative for natal and postnatal problems, for rheumatism and joint pains, increase lactation in feeding mothers, as antimicrobial, anti-inflammatory, antitumor agent, also used in diarrhea, dysentery, gonorrhea, leucorrhea etc. Its root contains steroidal and triterpenoidal saponins, sapogenins and fructans which act as therapeutic agents and play vital role in many therapeutic applications. It is a rich source of over 25 alkaloids, vitamins, proteins, carbohydrates, steroids, saponins, potassium, calcium, magnesium, phenol, resins, mucilage, and polysaccharides and also contains high quantity of simple sugars, mainly sucrose, glucose, fructose, galactose, mannose and xylose. The commercial exploitation of this plant and their secondary metabolites, germplasm conservation and in vitro production of secondary metabolites for quality control are some of the major prospects of this rare medicinal herb. The focus of the present review is to galvanize the potential of therapeutic and nutritive values of this herb and production of their secondary metabolites. The in vitro tuber induction, extraction, purification and characterization of saponins are also discussed in the present review. Keywords: Chlorophytum borivilianum, aphrodisiac agent, in vitro tuber induction, saponins, antioxidant.

Aging worms experience a decline in mobility [ 15 ], chemotaxis [ 16 ] and reproductive capacity [ 17 ], and become increasingly susceptible to lethal infections [ 18 ].

These features make C. Another model organism widely used in aging research is the budding yeast S. It has been shown that yeast cells have a finite replicative capacity, which is measurable by the number of their daughter cells.

In vitro multiplication of Chlorophytum borivilianum Sant. and Fern

Moreover, in a chronological lifespan, the length of time a yeast cell can survive in a non-dividing state, has been established as an additional read-out. Importantly, it has been shown that nutrient restriction can increase replicative the chronological lifespan in yeast [ 19 ]. The IIS is an evolutionarily conserved pathway that regulates life span across many species, one example being Drosophila melanogaster that, like C.

Therefore, experimental data obtained on simple model organisms could prove highly informative when mammals are considered [ 21 ].

In order to investigate the claims made in Ayurveda on the Indian Rasayana herb and its property to counteract the aging process and increase longevity, we first tested the polysaccharide of C. Additionally, we assessed in skin cell cultures and in a living human reconstructed epidermis if the same extract could improve parameters associated with aged skin. Materials and methods Plant material C. Powdered roots were extracted as aqueous decoction as per Ayurvedic Pharmacopoeia of India, using distilled water Ayurvedic Pharmacopoeia of India, Extraction of polysaccharides from the roots of C.

Extraction with de-mineralized hot water mL of the powdered roots 0. The resultant aqueous extract was centrifuged at 5,g for 10 minutes and then filtered using a glass filter GFD, G-3, Whatman filter paper. The C. The pellet formed under centrifugation represented an enriched polysaccharide.

The pellet was repeatedly washed sequentially with anhydrous ethanol 50 mL and acetone 50mL and further freeze-dried for 48h to obtain an amorphous white powder.

Carbohydrate and protein analysis The total carbohydrates were determined by the phenol-sulfuric acid assay with D-glucose as standard at nm [ 22 ]. Determination of amino acid composition was performed before and after protein hydrolysis using a dedicated Amino Acid Analyzer L Hitachi according to the methods by Guo et al [ 23 ].

The Hitachi L analyzer utilizes a lithium citrate buffer system and is optimized for physiological samples. After completion of hydrolysis, HCl in excess was removed and the tubes were vacuum-dried for 90 minutes. Assay of total amino acids colorimetric method The total amino acid content of protein free supernatants was estimated by a modified dinitrophenyl DNP derivatization method [ 24 ].

After adding 1 ml of 0. Apart from reagent blank and reference standards, some of the samples also contained a known amount of added glutamate:glycine mixture for assessing the recovery of total amino acids through the different steps of assay procedure.

Several data were obtained including the Mw values and the general formula using the Polytools software and the smart formula application. For the sample preparation, 1mg of the C.


To get a better co-crystallization, the deposits were quickly evaporated under air-flow. HRMS experiments. The samples were analyzed at a concentration of 0. This method allows oligosaccharide degree of polymerization DP to being estimated. The eluent was 0. Optical clarification of the solutions was achieved by filtering through 0. Plate preparation. The well lifespan analysis plates were filled with agar two days before bleaching.

Plates were left to dry off for 20 minutes under the laminar flow hood in sterile conditions followed by seeding feeding with fresh OP50 bacteria. Worm synchronization. On the same day when the compounds were added to the plates, the eggs were prepared by washing each of the stock 9cm-diameter plates with 3 ml of M9 buffer. Eggs were then collected by performing 1x five minutes centrifugation at 3, rpm, bleaching them with hypochlorite solution and finally centrifuging them twice at 2, rpm to remove dead worms and bacteria.

Chlorophytum in Flora of China @

The eggs-containing M9 solution was then diluted to the appropriate concentration to reach an average of 8, eggs per ml of M9 buffer solution. Plates were then dried-off under a laminar flow hood for approximately 10 minutes. Feeding with OP50 E. Lifespan analysis.

The lifespan analysis was performed using Sibelius proprietary software for processing imaging profiles into tabulated output of moving, live, and dead worms. Lifespan assay using S. The assay for chronological lifespan in yeast requires growth of cells to stationary phase where cells stop cell division but remain metabolically active [ 27 ]. H2O or vehicle containing various concentrations of the C. An O2 permeable membrane was used to prevent evaporation.

Ethics statement. Human dermal fibroblasts were isolated from mammary skin explants. The samples were anonymized before their reception by the authors. Only age, sex and anatomical site of samples were specified to the authors.

The authors did not participate in sample collection. This legislation does not require prior authorization by an ethics committee for sampling or use of surgical waste. Evaluation using human epidermal keratinocytes and dermal fibroblasts in monolayer culture Immortalized human epidermal keratinocyte cell line HaCaT obtained from Professor Dr.

Fusenig, Heidelberg, Germany as a kind gift and human dermal fibroblasts Caucasian were used for the experiments as described previously [ 28 ]. The polysaccharide was dissolved in DMSO vehicle for the treatment and evaluated using 96 well plates. The quantification of hyaluronic acid synthesis by HaCaT keratinocytes [ 29 ] was measured in the culture medium post five days incubation by ELISA [ 30 ].

Evaluation using human reconstructed epidermis model.

Chlorophytum borivilianum: A White Gold for Biopharmaceuticals and Neutraceuticals

The incidence of infertility, erectile dysfunction, subnormal desire and performance in sexual intercourse are increasing alarmingly. Approximately percent of all cohabiting couples are infertile. Up to 50 percent of these cases belong to male infertility, which means that nearly 7. Erectile dysfunction is said to afflict as much as 10 percent of the male population. Therefore, traditional medicines like Ayurveda and Chinese medicines have been the mainstay in this segment. However, owing to the associated hype and mystic, the activities of genuine herbs are subjected to criticism due to lack of scientific validation.

The answer to this pessimism lies in the appropriate scientific evaluation of the genuine herbs improving male sexual health. The root tuber of this small, annual herb is used for its versatile Shukrala beneficial effect on male sexual health , Rasayana adaptogenic activity , and Balya general health tonic properties. Owing to its marked clinical results, CB is being promoted as herbal Viagra. Experimental studies reaffirm its role in sexual behavior, spermatogenic activity,[ 4 , 5 ] immunomodulatory activity,[ 6 ] anti-stress and anti-oxidant activities.

Objective To assess the effect of the water extract of Chlorophytum borivilianum root tubers on semen and testosterone in healthy adult males.

Materials and Methods A randomized, double blind, placebo-controlled trial. Study population The study population was made up of apparently healthy and consenting male volunteers between 20 to 40 years of age, registered at the OPD of NIA, Jaipur. The total number of 30 volunteers were registered after taking due consent and were randomly assigned to two groups A and B. Trial drug Botanically authenticated dried root tubers of C. Water extract of the quality-assured samples was prepared and capsulated in soft-gelatin capsules of mg each.

Barley powder was encapsulated in similar capsules as placebo in the study dose, because it had practically no effect on semen and testosterone. Dose, duration, and administration The trial drug was given as capsules of mg, twice daily, before food, with normal water, for 12 weeks.